Analysis Note

Positive ControlEndothelial cells

Application

Competition Studies (see comments)r>ELISA (0.5-1 µg/ml)r>Immunoblotting (0.1-0.2 µg/ml)r>Paraffin Sections (5-15 µg/ml)

General description

This Anti-VEGFR1 Goat pAb is validated for use in Competition Studies, ELISA, Immunoblotting, Paraffin Sections for the detection of VEGFR1.

Recognizes VEGF receptor 1.

Immunoaffinity purified goat polyclonal antibody. Recognizes the VEGF receptor 1 protein.

Immunogen

recombinant, human VEGFR1 (extracellular domain)/Fc chimera

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.

Soker, S., et al. 1998. Cell92, 735.Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem.272, 32521.Kendall, R.L. and Thomas, K.A. 1993. PNAS.90, 10705.Klagsbrun, M. and Soker, S. 1993. Current Biology3, 699.Senger, D., et al. 1993. Cancer and Metastasis Rev.12, 303.Brown, L., et al. 1992. Kidney International42, 1457.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from a sterile solution in PBS, 5% trehalose.

Reconstitution

Reconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)